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Bioengineering

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JoVE Journal Bioengineering
Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue
 

Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue

Article DOI: 10.3791/60932-v • 06:33 min • May 17th, 2021
May 17th, 2021 •
*5, *1, 1, 1, 1, 2, 3,4, 1
1Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, 2Department of General Surgery, Nanfang Hospital, Southern Medical University, 3Department of Stomatology, Nanfang Hospital, Southern Medical University, 4College of Stomatology, Southern Medical University, 5School of Laboratory Medicine and Biotechnology, Southern Medical University
* These authors contributed equally

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In this experiment, a mouse is injected in its tail vein with Rhodamine B isothiocyanate–dextran that can stain blood vessels. After the liver is exposed and fixed, a specific part of the liver can be selected to observe the deep tissue in the living body using multiphoton microscopy.

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Multiphoton Microscopy Vessels Mouse Liver Tissue Visualization Blood Vessels Dynamics Three Dimensions Vascular Dye Staining Microscopic Visualization Anesthetized Mouse Tail Injection Rhodamine B Isothiocyanate-Dextran Caudal Tail Vein Puncture Site Abdomen Shaving Heating Pad Organ Imaging Frame
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