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An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats
DOI:

09:25 min

May 08, 2020

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Chapitres

  • 00:04Introduction
  • 00:53Heart Explantation and Perfusion
  • 03:13Heart Implantation
  • 06:24Enzymatic Heart Digestion and Subcutaneous Cardiac Cell Injection
  • 08:08Results: Representative Analyses of the Specificity of the Immune Response in Heterotopic Heart Transplant Rejection
  • 09:00Conclusion

Summary

Traduction automatique

We describe a model of heterotopic abdominal heart transplantation in rats, implying modifications of current strategies, which lead to a simplified surgical approach. Additionally, we describe a novel rejection model by in-ear injection of vital cardiac muscle cells, allowing further transplant immunological analyses in rats.

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