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Vurdering af den aldersspecifikke fagocytisk evne til voksen Drosophila melanogaster hemocytter ved hjælp af en In Vivo Phagocytosis Assay
Journal JoVE
Biologie
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Journal JoVE Biologie
Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

Vurdering af den aldersspecifikke fagocytisk evne til voksen Drosophila melanogaster hemocytter ved hjælp af en In Vivo Phagocytosis Assay

DOI:
10.3791/60983-v

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05:20 min

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June 11, 2020

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, ,
1Department of Biology,University of Maryland Baltimore County

Chapitres

  • 00:04Introduction
  • 00:36Injection of Flies
  • 02:03Dorsal Vessel Dissection
  • 03:07Fixation, Mounting, and Processing
  • 03:50Results: Imaging and Quantification of Phagocytic Events
  • 04:36Conclusion

Summary

Traduction automatique

Traduction automatique

Denne protokol beskriver en in vivo-analyse af fagocytose, der anvendes til at vurdere og kvantificere unges og alderen drosophila melanogastereøges evne til fagocytosebakterier..

Tags

Phagocytic AbilityHemocytesDrosophila MelanogasterIn Vivo Phagocytosis AssayPhagocytic EventsDissectionGlass NeedlesInjectionFluoro ParticlesPH-sensitive ParticlesStereomicroscopeInsect PinsDissection MediaCuticle ScissorsForcepsDorsal Vessel

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