Journal
/
Neurosciences
/
Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

DOI:
10.3791/60993-v

•

10:12 min

•

May 05, 2020

•

, , , ,
1Department of Physiology and Pharmacology, Sackler Faculty of Medicine,Tel Aviv University, 2Sagol School of Neuroscience,Tel Aviv University

Chapitres

  • 00:04Introduction
  • 01:08Microfluidic Chamber Preparation
  • 05:53Neuronal Culture Plating
  • 07:24Axonal Transport
  • 08:19Results: Axonal Transport of Mitochondria and Acidic Compartments in Motor Neurons
  • 09:34Conclusion

Summary

Traduction automatique

Traduction automatique

Axonal transport is a crucial mechanism for motor neuron health. In this protocol we provide a detailed method for tracking the axonal transport of acidic compartments and mitochondria in motor neuron axons using microfluidic chambers.

Tags

Axonal TransportMotor NeuronMicrofluidic ChambersNeurodegenerative DiseaseALSAxonal GrowthAxonal DegenerationPDMSChlorotrimethylsilaneVacuum Desiccator

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/60993?article_type=v

Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
check_url/fr/60993?article_type=v

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues
check_url/fr/60993?article_type=v

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System
check_url/fr/60993?article_type=v

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
check_url/fr/60993?article_type=v

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
check_url/fr/60993?article_type=v

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
check_url/fr/60993?article_type=v

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
check_url/fr/60993?article_type=v

A Simple Neuronal Mechanical Injury Methodology to Study Drosophila Motor Neuron Degeneration
check_url/fr/60993?article_type=v

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration
check_url/fr/60993?article_type=v

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Read Article