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Målinger af fysiologiske stressreaktioner i C. Elegans
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Journal JoVE Biologie
Measurements of Physiological Stress Responses in C. Elegans

Målinger af fysiologiske stressreaktioner i C. Elegans

DOI:
10.3791/61001-v

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10:36 min

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May 21, 2020

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, , , , , , ,
1Department of Molecular and Cell Biology,University of California, Berkeley

Chapitres

  • 00:04Introduction
  • 01:00Stress Response Induction: hsp-4p::GFP Readout
  • 02:11Stereo Microscope or Low-Magnification Wide-Field/Compound Microscope Imaging
  • 04:33Quantitative Reporter Measurement
  • 06:54Stress and Heat Sensitivity Measurement
  • 07:54Results: Representative Cellular Stress Response Evaluation
  • 09:56Conclusion

Summary

Traduction automatique

Traduction automatique

Her karakteriserer vi cellulære proteotoksiske stressreaktioner i nematoden C. elegans ved at måle aktiveringen af fluorescerende transskriptionelle journalister og assaying følsomhed over for fysiologisk stress.

Tags

C. ElegansPhysiological Stress ResponsesStress Response AssayHeat Shock ProteinEndoplasmic Reticulum Unfolded Protein ResponseGFP ReporterSodium AzideMicromanipulationHigh-throughputGenetic PerturbationsPharmacological PerturbationsMolecular And Physiological AnalysisSynchronizationImagingMicroscopy

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