Journal
/
Neurosciences
/
Induction of Leptomeningeal Cells Modification Via Intracisternal Injection
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Induction of Leptomeningeal Cells Modification Via Intracisternal Injection

Induction of Leptomeningeal Cells Modification Via Intracisternal Injection

DOI:
10.3791/61009-v

•

05:55 min

•

May 07, 2020

•

, ,
1Department of Cell and Molecular Biology,Karolinska Institute

Chapitres

  • 00:04Introduction
  • 01:07Preparation of the Injection System
  • 02:54Intracisternal Injection
  • 03:55Results: Specific Labelling of Leptomeningeal Cells via Intracisternal Injection of Endoxifen
  • 05:07Conclusion

Summary

Traduction automatique

Traduction automatique

We describe an intracisternal injection that employs a needle bent at the tip that can be stabilized to the skull, thus eliminating the risk of damage to the underlying parenchyma. The approach can be used for genetic fate mapping and manipulations of leptomeningeal cells and for tracking cerebrospinal fluid movement.

Tags

Keywords: Leptomeningeal CellsIntracisternal InjectionGene EditingNeurodevelopmentBacterial MeningitisCerebrospinal Fluid FlowHamilton SyringeEndoxifenIsofluraneCisterna MagnaDural MembraneSurgical Procedure

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/61009?article_type=v

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
check_url/fr/61009?article_type=v

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
check_url/fr/61009?article_type=v

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
check_url/fr/61009?article_type=v

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
check_url/fr/61009?article_type=v

An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
check_url/fr/61009?article_type=v

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
check_url/fr/61009?article_type=v

Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy
check_url/fr/61009?article_type=v

Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation
check_url/fr/61009?article_type=v

Unveiling Cellular Functions and Potential Clinical Implications of Leptomeningeal Lymphatic Endothelial Cells (Video) | JoVE
check_url/fr/61009?article_type=v

Applications of TEER Detection to Assess Cell Barrier Integrity (Video) | JoVE

Read Article