/
/
Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:04Introduction
- 00:3748 Hours Post Fertilization (HPF) Zebrafish Embryo Dechorionation
- 01:42Dithiothreitol (DTT) Treatment
- 02:17Embryo Dissociation
- 03:13Flow Cytometric Sample Preparation
- 03:51Flow Cytometry
- 06:10Results: Representative Effects of Morpholino (MO)-Mediated Transcript Reduction on Red Blood Cell Production Numbers During Embryogenesis
- 07:01Conclusion
This assay is a simple method to quantitate hematopoietic cells in developing embryonic zebrafish. Blood cells from dissociated zebrafish are subjected to flow cytometry analysis. This allows the detection of blood defects in mutant animals and after genetic modification.
