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Flow Cytometric Analyse av lymfocytt infiltrasjon i sentralnervesystemet under eksperimentell autoimmun encefalomyelitt
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1Translational Medicine Research Center, Ruijin Hospital North,Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology,Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation,Shanghai Jiao Tong University School of Medicine
Chapitres
- 00:04Introduction
- 00:53C57BL/6 Mouse Immunization
- 01:58Single Brain Cell Suspension Preparation
- 04:19Flow Cytometric Analysis
- 07:00Results: Representative Experimental Autoimmune Encephalomyelitis (EAE) Course and Brain Lymphocyte Analyses
- 08:22Conclusion
Dette manuskriptet presenterer en protokoll for å indusere aktiv eksperimentell autoimmun encefalomyelitt (EAE) hos mus. En metode for isolasjon og karakterisering av infiltrerte lymfocytter i sentralnervesystemet (CNS) presenteres også for å vise hvordan lymfocytter er involvert i utviklingen av CNS autoimmun sykdom.
