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Biochemistry

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JoVE Journal Biochemistry
En elektrokæminescensbaseret analyse af MeCP2-proteinvarianter
 
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En elektrokæminescensbaseret analyse af MeCP2-proteinvarianter

Article DOI: 10.3791/61054-v • 07:44 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 2, 3, 1, 1, 1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

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Elektrochemiluminescensensimmunoassay (ECLIA) er en ny metode til kvantitativ påvisning af endogene og eksogent anvendte MeCP2-proteinvarianter, som producerer meget kvantitative, præcise og reproducerbare målinger med lav intra- og analysefejl over et bredt arbejdsområde. Her er protokollen for MeCP2-ECLIA i et 96-godt format beskrevet.

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Biokemi Udgave 159 ECLIA Sulfo-TAG MeCP2 TAT fusionsprotein TAT-MeCP2 Rett syndrom proteinsubstitutionsterapi
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