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Journal / Developmental Biology / 为早期小鼠胚胎测序准备小型RNA库
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Developmental Biology

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JoVE Journal Developmental Biology
为早期小鼠胚胎测序准备小型RNA库
 
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为早期小鼠胚胎测序准备小型RNA库

Article DOI: 10.3791/61086-v • 08:37 min • October 9th, 2020
October 9th, 2020 •
1, 1
1Development, Disease Models & Therapeutics Graduate Program, Department of Molecular and Cellular Biology, Department of Neuroscience, Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center, Baylor College of Medicine

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我们描述了一种在早期小鼠胚胎中分析微RNA的技术。该协议克服了低细胞输入和小RNA富集的挑战。此测定可用于分析早期小鼠胚胎不同细胞系中 miRNA 表达随时间变化。

Tags

发育生物学，第164期，神经波峰，微RNA，小RNA测序，发育时间，转录后调控，小鼠
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