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Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
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Journal JoVE Biologie
Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

DOI:
10.3791/61100-v

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09:18 min

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September 07, 2021

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, ,
1Department of Biomedical Genetics,University of Rochester Medical Center

Chapitres

  • 00:04Introduction
  • 01:09Reagent Preparation
  • 02:19Synchronization and Inhibiting Progeny Production
  • 04:05Measuring Decline in Proteostasis
  • 06:39Results: HPK-1 Promotes Proteostasis
  • 08:37Conclusion

Summary

Traduction automatique

Traduction automatique

Proteostatic decline is a hallmark of aging, facilitating the onset of neurodegenerative diseases. We outline a protocol to quantifiably measure proteostasis in two different Caenorhabditis elegans tissues through heterologous expression of polyglutamine repeats fused to a fluorescent reporter. This model allows rapid in vivo genetic analysis of proteostasis.

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Keywords: ProteostasisCaenorhabditis ElegansPolyglutamine Fluorescent ReporterAgingProtein Misfolding DiseasesAlzheimer’sParkinson’sHuntington’s DiseaseAmyotrophic Lateral SclerosisRNAiOP50 E.coliHypochlorite TreatmentL1 Animals

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