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Immunologie et infection
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Optimization of the Cuff Technique for Murine Heart Transplantation
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Immunologie et infection
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Journal JoVE Immunologie et infection
Optimization of the Cuff Technique for Murine Heart Transplantation

Optimization of the Cuff Technique for Murine Heart Transplantation

DOI:
10.3791/61103-v

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14:01 min

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June 26, 2020

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, , , , , , , ,
1Organ Transplantation Institute, School of Medicine,Xiamen University, 2Fujian Key Laboratory of Organ and Tissue Regeneration, 3Department of Kidney Transplantation, Center of Organ Transplantation,The Second Xiangya Hospital of Central South University, 4Clinical Research Center for Organ Transplantation in Hunan Province, 5Clinical Immunology Center,Central South University, 6School of Medicine,Xiamen University, 7Xiamen Medical College, 8Medical College,Guangxi University

Chapitres

  • 00:01Introduction
  • 01:32Recipient Preparation
  • 07:06Donor Preparation
  • 11:35Representative Results
  • 13:39Conclusion

Summary

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We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.

Tags

Cuff TechniqueCardiac TransplantationSurgical EfficiencyTube InsertionThermal Surgeon Operation ModuleExternal Jugular VeinCarotid ArteryBlunt DissectionMicro ForcepsSuture

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