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Aislamiento de células endoteliales endocardiales y coronarias de la pared libre ventricular del corazón de rata
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1Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, 3Stanford Cardiovascular Institute,Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center
Chapitres
- 00:04Introduction
- 01:11Heart Isolation
- 02:34Endocardial Endothelial Cell (EEC) Digestion
- 03:34Coronary Endothelial Cell (CEC) Digestion
- 04:36Endothelial Cell (EC) Sorting
- 06:56Results: Representative qPCR Data CEC and EEC Isolation Verification
- 07:46Conclusion
Presentamos un protocolo para el aislamiento de células endoteliales endocardiales y coronarias de corazones de rata a través de la digestión secuencial del tejido en un tampón de digestión, la recolección celular de ciclos de centrífuga recurrentes y la purificación celular mediante microperlas CD31 anti-rata.
