Journal
/
Bio-ingénierie
/
Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining
Journal JoVE
Bio-ingénierie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Bio-ingénierie
Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining

Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining

DOI:
10.3791/61143-v

•

10:40 min

•

June 13, 2020

•

, , , , , , , , ,
1iBB – Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico,Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina,Universidade de Lisboa, 3The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus,Universidade de Lisboa, 4PBS Biotech, Inc, Camarillo, CA, USA

Chapitres

  • 00:04Introduction
  • 00:23Seeding the Bioreactor
  • 02:06Differentiation and Maturation of Aggregates
  • 03:26Preparing Organoids for Cryosectioning
  • 05:47Immunostaining
  • 08:27Results: Efficient Cerebellar Differentiation and Maturation
  • 10:09Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes a dynamic culture system to produce controlled size aggregates of human pluripotent stem cells and further stimulate differentiation in cerebellar organoids under chemically-defined and feeder-free conditions using a single-use bioreactor.

Tags

Keywords: Cerebellar OrganoidsHuman Pluripotent Stem CellsScalable GenerationSingle-use BioreactorsImmunostainingIPSC AggregatesGfCDM Differentiation MediumCell DetachmentCentrifugationCell CountingBioreactor CultivationMedium ExchangeOrganoid Storage

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/61143?article_type=v

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
check_url/fr/61143?article_type=v

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
check_url/fr/61143?article_type=v

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System
check_url/fr/61143?article_type=v

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
check_url/fr/61143?article_type=v

Growth and Characterization of Irradiated Organoids from Mammary Glands
check_url/fr/61143?article_type=v

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
check_url/fr/61143?article_type=v

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions
check_url/fr/61143?article_type=v

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
check_url/fr/61143?article_type=v

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
check_url/fr/61143?article_type=v

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

Read Article