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1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove,University of Defence, 2Department of Clinical Biochemistry and Diagnostics,University Hospital, 3Department of Oncology, Thomayer Hospital,Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine,Charles University
Chapitres
- 00:04Introduction
- 00:40Cell Culture for Immunocytochemistry Staining
- 03:10Immunofluorescent Staining of Primary Cilia In Vitro
- 06:11Results: Fluorescent Imaging of Primary Cilia in Fibroblasts
- 07:26Conclusion
Primer silia centriole ile ilişkili hücre dışı yapılardır. İmmünfloresan boyama ile primer silia tespiti son derece yüksek kaliteli görüntülerle sonuçlanan nispeten basit bir işlemdir. Bu protokolde primer silya yı ifade eden fibroblastlar sabit, immünolekeli ve floresan veya konfokal mikroskopta görüntülendi.
