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Improved Fecundity and Fertility Assay for Aedes aegypti using 24 Well Tissue Culture Plates (EAgaL Plates)
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Journal JoVE Biologie
Improved Fecundity and Fertility Assay for Aedes aegypti using 24 Well Tissue Culture Plates (EAgaL Plates)

Improved Fecundity and Fertility Assay for Aedes aegypti using 24 Well Tissue Culture Plates (EAgaL Plates)

DOI:
10.3791/61232-v

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06:27 min

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May 04, 2021

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,
1Department of Entomology,Texas A&M University

Chapitres

  • 00:04Introduction
  • 00:38Plate Preparation and Mosquito Feeding
  • 02:01Ovipositioning and Egg Counting
  • 04:25Fertility Assessment
  • 05:22Results: FeT Gene Silencing
  • 05:57Conclusion

Summary

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Described is a time and space-saving method to count eggs and determine hatch rates of individual mosquitoes using 24 well tissue culture plates, which can substantially increase the scale and speed of fecundity and fertility assays.

Tags

Aedes AegyptiFecundityFertility AssayEAgaL PlatesOviposition ExperimentAgaroseBlood FeedingEgg DevelopmentEgg Counting

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