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Billeddannelse og analyse af olierøde O-farvede hele Aorta-læsioner i en aneurismehyperlipidæmi-musemodel
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Médecine
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Journal JoVE Médecine
Imaging and Analysis of Oil Red O-Stained Whole Aorta Lesions in an Aneurysm Hyperlipidemia Mouse Model

Billeddannelse og analyse af olierøde O-farvede hele Aorta-læsioner i en aneurismehyperlipidæmi-musemodel

DOI:
10.3791/61277-v

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07:57 min

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May 02, 2022

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, ,
1Yale Cardiovascular Research Center, Department of Internal Medicine,Yale University School of Medicine, 2Department of Surgery,Yale University School of Medicine, 3Department of Cell Biology,Yale University School of Medicine

Chapitres

  • 00:05Introduction
  • 00:38Heart Perfusion
  • 01:39Aorta and Aortic Branch Isolation
  • 02:36Heart and Aorta Fixation
  • 03:29Unopened Whole Aorta Oil Red O Staining and Imaging
  • 04:43En Face Aorta Mounting
  • 05:39Results: Representative Atherosclerotic Lesion Visualization and Quantification
  • 06:57Conclusion

Summary

Traduction automatique

Traduction automatique

Denne protokol giver en trinvis procedure til analyse af aterosklerotisk byrde hos mus. Efterforskere kan bruge denne protokol til at sammenligne overflod, placering og størrelse af aterosklerotiske læsioner hos forskellige dyr.

Tags

AortaAtherosclerosisAneurysmHyperlipidemiaMouse ModelOil Red O StainingPerfusionDissectionFixationQuantificationVascular Lesions

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