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힘줄 유래 줄기 세포의 세포 분화를 유도하기 위해 3차원 동축 기계 자극 생물 반응기 시스템을 적용
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Médecine
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Journal JoVE Médecine
Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells
DOI:

14:04 min

August 01, 2020

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Chapitres

  • 00:00Introduction
  • 02:33Isolation of Mice TDSCs
  • 03:30Culture and Expansion of Mice TDSCs
  • 05:12Preparation of Stimulation Culture Medium for Cell Sheet Formation
  • 05:44Cell Sheet Formation by Culturing in Stimulation Medium
  • 06:21Preparation of 3D Tendon Stem Cell Construct
  • 07:50Assembly of the Uniaxial-Stretching Mechanical Stimulation Complex in Unique designed Bioreactor
  • 09:25Evaluation of the Mechanical Stimulated in vitro Tendon-like Tissue
  • 10:10Representative Results
  • 11:07Discussion

Summary

Traduction automatique

3차원 단방향 기계적 자극 생물반응기 시스템은 힘줄 유래 줄기 세포 및 신힘주 형성의 테노겐 특이적 분화를 위한 이상적인 생물반응기입니다.

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