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Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos
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1Integrative Molecular and Biomedical Sciences,Baylor College of Medicine, 2Department of Cell and Molecular Biology, School of Molecular and Cellular Biology,University of Illinois at Urbana-Champaign, 3Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor College of Medicine
Chapitres
- 00:04Introduction
- 00:51Embryo Collection and Mounting
- 03:43Injection and Heat Stress to Promote Rod Formation
- 04:45Imaging of Actin Rods with Confocal Microscopy
- 06:32Results: Actin Rods in Heat-stressed Embryos
- 07:17Conclusion
Tags
Keywords:
Intranuclear Actin RodsLive Heat Stressed Drosophila EmbryosMicroinjectionActin Stress ResponseActin Rod AssemblyMutant ScreenStress ConditionsEmbryo HandlingEmbryo PositioningEmbryo MountingG-actin RedApple Juice AgarDechorionationEmbryo TransferEmbryo AlignmentEmbryo GlueCoverslip Mounting
