Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology,Jagiellonian University in Krakow, 2Department of Histology,Jagiellonian University Medical College, 3Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Faculty of Biology,Jagiellonian University in Krakow
Chapitres
- 00:04Introduction
- 01:03Isolation of Porcine Cumulus-oocyte Complexes (COCs)
- 02:35Encapsulation in Fibrin-alginate Hydrogel Beads
- 04:48Encapsulation in Super-hydrophobic Fluorinated Ethylene Propylene (FEP) Microbioreactors
- 06:17Results: Morphology and Viability of COCs after Encapsulation
- 07:15Conclusion
Præsenteret her er to protokoller for indkapsling af svin oocytter i 3D-kultur betingelser. I den første er cumulus-oocyt komplekser (P-piller) indkapslet i fibrin-alginat perler. I den anden, de er omgivet af fluorerede ethylen propylen pulverpartikler (mikrobioreaktorer). Begge systemer sikrer optimale betingelser for at opretholde deres 3D-organisation.
