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A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures
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Chapitres
- 00:04Introduction
- 00:49Rhinal Cortex-Hippocampus Slice Preparation
- 03:41Electrophysiological Recordings
- 05:07Propidium Iodide (PI) Uptake Assay and Immunohistochemistry
- 07:26Results: Epileptic-like Events, Neuronal Death and Gliosis are Observed in Rhinal Cortex-Hippocampus Organotypic Slices
- 09:18Conclusion
Here, we describe the preparation of rhinal cortex-hippocampus organotypic slices. Under a gradual and controlled deprivation of serum, these slices depict evolving epileptic-like events and can be considered an ex vivo model of epileptogenesis. This system represents an excellent tool for monitoring the dynamics of spontaneous activity, as well as for assessing the progression of neuroinflammatory features throughout the course of epileptogenesis.
