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En bakteriell oral fôringsanalyse med antibiotikabehandlede mygg
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Biologie
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Journal JoVE Biologie
A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

En bakteriell oral fôringsanalyse med antibiotikabehandlede mygg

DOI:
10.3791/61341-v

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09:59 min

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September 12, 2020

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, , , , , ,
1Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science,Hunan Normal University, 2State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science,Hunan Normal University, 3Department of Infectious Diseases, the Second Xiangya Hospital,Central South University

Chapitres

  • 00:00Introduction
  • 01:30Midgut Dissection and Cultivable Bacteria Isolation
  • 04:55Antibiotic Treatment and Bacterium Reintroduction
  • 09:14Representative Results

Summary

Traduction automatique

Traduction automatique

Denne artikkelen presenterer en protokoll for å undersøke effekten av individuelle myggtarmet bakterier, inkludert isolasjon og identifisering av mygg midgut kulterbare mikrober, antibiotikauttømming av myggtarmbakterier og gjeninnføre en bestemt bakterieart.

Tags

Bacterial Oral Feeding AssayAntibiotic-treated MosquitoesMosquito MidgutGut Microbiota16S RRNA Gene SequencingAntibiotic TreatmentColony Forming Unit AssayMosquito PhysiologyMidgut DissectionBacterial IsolationSurface Sterilization

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