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Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

DOI:
10.3791/61363-v

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06:10 min

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May 13, 2020

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,
1Department of Biological Sciences,Lehigh University

Chapitres

  • 00:04Introduction
  • 00:41Thorax Isolation and Fixation
  • 02:12Flash Freezing and Thorax Bisection
  • 03:41Structural Staining
  • 04:08Tissue Mounting
  • 04:57Results: Representative Synaptic Integrity Assessment
  • 05:46Conclusion

Summary

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The goal of this procedure is to dissect the dorsal longitudinal muscle (DLM) tissue to assess the structural integrity of DLM neuromuscular junctions (NMJs) in neurodegenerative disease models using Drosophila melanogaster.

Tags

Synaptic DegenerationAdult DrosophilaNeurodegenerationNeuromuscular JunctionsALSParkinson’sHuntington’sAlzheimer’s DiseaseDissectionAnesthesiaForcepsDissection ScissorsFormaldehydeCryoprotective GlovesLiquid NitrogenFeather BladePasteur Pipette

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