This content is Free Access.
Chapitres
- 00:04Introduction
- 00:28Large-Scale Culture Plate (LSCP) Gravid Adult Spot Bleaching
- 01:21LSCP Sample Harvest
- 03:02Population Size Estimation
- 04:19Large Particle Flow Cytometry (LPFC) Preparation
- 05:27Population Distribution Documentation and 384-Well Imaging Plate Preparation
- 06:24Results: Representative Large-Scale Mixed-Stage C. elegans Population Evaluation
- 08:06Conclusion
To use Caenorhabditis elegans (C. elegans) in omics research, a method is needed to generate large populations of worms where a single sample can be measured across platforms for comparative analyses. Here, a method to culture C. elegans populations on large-scale culture plates (LSCPs) and to document population growth is presented.
