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A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury
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Biologie
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Journal JoVE Biologie
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

DOI:
10.3791/61455-v

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09:50 min

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June 17, 2020

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, , , , , , , ,
1Department of Urology,Boston Children’s Hospital, 2Department of Surgery,Harvard Medical School, 3Division of Hematology/Oncology,Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences,Harvard Medical School, 7Vascular Biology Program,Boston Children’s Hospital

Chapitres

  • 00:04Introduction
  • 00:57Mouse Preparation
  • 01:48Spinal Cord Injury (SCI)
  • 03:17Tissue Perfusion and Procurement
  • 04:34Cell Dissociation and Single Cell Suspension Preparation
  • 05:59Cell Staining
  • 06:44Flow Cytometry Analysis
  • 07:43Results: Representative Mouse Bladder Collagen Expression and Immune Cell Population Analyses
  • 08:54Conclusion

Summary

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Traduction automatique

The goal of this protocol is to apply an optimized tissue dissociation protocol to a mouse model of spinal cord injury and validate the approach for single cell analysis by flow cytometry.

Tags

Keywords: Single Cell DissociationMolecular AnalysisUrinary BladderMouseSpinal Cord InjuryFACS AnalysisLaminectomySpinal Cord TransectionBladder Expression

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