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Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
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Journal JoVE Biologie
Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

DOI:
10.3791/61461-v

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06:46 min

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June 24, 2020

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, , , , ,
1Maria Sklodowska-Curie National Research Institute of Oncology

Chapitres

  • 00:04Introduction
  • 00:33Confluent Monolayer Generation
  • 01:06Focal Adhesion Analysis
  • 03:13Manual Cell Shape Analysis
  • 04:04Automated Cell Shape Analysis
  • 04:54Results: Representative Focal Adhesion and Cell Shape Distribution Analyses
  • 06:18Conclusion

Summary

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The article describes quantification of 1) the size and number of focal adhesions and 2) cell shape index and its distribution from confocal images of the confluent monolayers of MCF7 cells.

Tags

Cell-substrate AdhesionCell ShapeMCF7 Cell MonolayersQuantificationConfocal MicroscopyImageJFocal Adhesion AnalysisBackground SubtractionBinary ConversionParticle AnalysisImageJ Macro

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