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Stabilitet och struktur av Fladdermus Major Histocompatibility Complex Klass I med heterologous β2-Microglobulin
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1School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences,University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Academy of Sciences, 5Savaid Medical School,University of Chinese Academy of Sciences
Chapitres
- 00:04Introduction
- 00:54E. coli Transformation
- 01:46Culture Inoculation
- 02:59Bacteria Harvest
- 03:28Inclusion Body Purification
- 04:27MHC Complex Refolding
- 04:53MHC H Chain and Beta2m Injection and Dilution
- 05:42Refolded Protein Concentration Determination
- 06:51Crystallization, Data Collection, and Processing
- 07:41Structure Determination and Analyses
- 08:33Results: Representative Bat MHC I Beta2-Microglobulin Stability and Structure Analysis
- 10:21Conclusion
Protokollet beskriver experimentella metoder för att erhålla stabila stora histocompatibility komplexa (MHC) klass I genom potentiella β2-mikroglobulin(β2m) substitutioner från olika arter. Den strukturella jämförelsen av MHC jag stabiliseras av homologa och heterologa β2m undersöktes.
