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Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU
Journal JoVE
Biologie
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Journal JoVE Biologie
Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

DOI:
10.3791/61483-v

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08:37 min

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September 09, 2020

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, , , , , , , ,
1Laboratorio de Neurociencias, Departamento de Psicología,Universidad Iberoamericana Ciudad de México, 2Facultad de Ciencias,Universidad Autónoma del Estado de México

Chapitres

  • 00:04Introduction
  • 01:06Thymidine Analog (5‐Bromo‐2’‐Deoxyuridine) BrdU Administration and Tissue Preparation
  • 02:02BrdU Detection
  • 04:40Cell Quantification
  • 05:33Image Deconvolution
  • 06:59Results: BrdU‐Labeled Cell Identification and Quantification in Representative DG Tissue Section
  • 07:46Conclusion

Summary

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This paper presents four of the most common techniques for visualizing BrdU positive cells to measure adult neurogenesis in rats. This work includes instructions for reagent preparation, thymidine analog administration, transcardial perfusion, tissue preparation, peroxidase immunohistochemical reaction, immunofluorescence, signal amplification, counterstaining, microscopy imaging, and cell analysis.

Tags

ImmunohistochemistryCellular ProliferationNeurogenesisBrdUThymidine AnalogPermeabilizationDNA DenaturationAntibody StainingCryosectioningPeroxidase ReactionAvidin-biotin-peroxidase Complex

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