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直接耦合电雷电图（DC-ERG），用于记录小鼠视网膜颜料皮皮的光唤起电响应
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Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
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直接耦合电雷电图（DC-ERG），用于记录小鼠视网膜颜料皮皮的光唤起电响应

DOI:
10.3791/61491-v

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09:10 min

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July 14, 2020

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, , , ,
1Retinal Neurophysiology Section, National Eye Institute,National Institutes of Health, 2Ocular and Stem Cell Translational Research Section, National Eye Institute,National Institutes of Health, 3Human Visual Function Core, National Eye Institute,National Institutes of Health

Chapitres

  • 00:04Introduction
  • 00:44Capillary Electrode Filling
  • 01:51Microelectrode Holder Preparation
  • 02:46Electrode Test
  • 04:08Mouse and Electrode Positioning
  • 06:15Direct-Coupled Electroretinogram (DC-ERG) Recording
  • 07:04Results: Representative DC-ERG Traces and Workflow Analyses
  • 08:25Conclusion

Summary

Traduction automatique

Traduction automatique

在这里，我们提出了一种方法，使用马莫斯坦、佩奇和同事在21世纪早期首次描述的一种称为DC-ERG的技术，记录小鼠视网膜色素上皮（RPE）的光唤起电反应。

Tags

Direct-coupled ElectroretinogramDC-ERGRetinal Pigment EpitheliumNon-invasive EvaluationAge-related ChangesDisease ProgressionPharmacological InterventionReproducibilityCapillary ElectrodeHBSSVacuum ChamberCustom StandMicroelectrode HolderT-clipMagnetic Ball JointElectrode TestGround ElectrodeReference Electrode

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