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1Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research,Centro Médico Nacional 20 de Noviembre, ISSSTE, 2Vascular Surgery and Angiology Department,Centro Médico Nacional 20 de Noviembre, ISSSTE, 3Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research,Centro Médico Nacional 20 de Noviembre, ISSSTE
Chapitres
- 00:04Introduction
- 01:33Flow-Mediated Dilation (FMD) Evaluation
- 02:11Lower Limb Vascular Blood Evaluation, Blood Sampling, and Balloon Angioplasty
- 04:07Circulating Mononuclear Progenitor Cell (MPC) Isolation and Antibody Staining
- 05:21MPC Quantification
- 06:10Results: Representative Relationship Between MPC and Hemodynamic Indicators and Lower Limb Amputation Prognosis After Angioplasty
- 07:04Conclusion
Lower limb amputation may occur even after angioplasty of obstructed vessels in Critical Limb Ischemia (CLI). Mononuclear Progenitor Cells (MPCs) reflect vascular repair. The present protocol describes the quantification of MPCs from circulation close to angioplasty, and its relationship with endothelial dysfunction and prediction of lower limb amputation.
