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Oppnå primære osteocytter gjennom murine calvarial fraksjonering av GFP-uttrykkende osteocytter
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JoVE Journal Biology
Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes

Oppnå primære osteocytter gjennom murine calvarial fraksjonering av GFP-uttrykkende osteocytter

DOI:
10.3791/61513-v

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07:22 min

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June 02, 2020

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, , , , , , , , , ,
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine,Tohoku University Graduate School of Dentistry

Chapters

  • 00:05Introduction
  • 00:44Newborn Mouse Calvaria Dissection
  • 01:38Newborn Mouse Calvaria Fractionation
  • 03:47Osteocyte Preparation for Fluorescence Activated Cell Sorting
  • 05:13Results: Representative Osteocyte Characterization
  • 06:44Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver disseksjon av neonatal dmp1-topaz mus calvaria og isolering av osteocytter som uttrykker det grønne fluorescerende proteinet gjennom cellefordøyelse og fraksjonering, i tillegg til osteocytpreparasjon for fluorescensaktivert cellesortering (FACS).

Tags

Primary OsteocytesCalvarial FractionationGFP-expressing OsteocytesOsteocyte IsolationCell CultureCollagenaseEDTACell FractionationCell Purification

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