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Analyse van somatische hypermutatie in de JH4-intron van Germinal Center B-cellen van Mouse Peyer's Patches
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches
DOI:

09:35 min

April 20, 2021

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Chapitres

  • 00:05Introduction
  • 01:03Dissection of Peyer’s Patches
  • 02:25Cell Isolation and Staining for FACS
  • 05:12JH4 Intron Sequence Amplification and Analysis
  • 07:39Results: Flow Cytometry and JH4 Sequence Analysis
  • 08:52Conclusion

Summary

Traduction automatique

Hier wordt een test gepresenteerd om somatische hypermutatie binnen de immunoglobuline zware keten gen locus te kwantificeren met behulp van germinale centrum B cellen van muis Peyer's patches.

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