Journal
/
/
Super-Resolution Live Cell Imaging of Subcellular Structures
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Super-Resolution Live Cell Imaging of Subcellular Structures
DOI:

06:50 min

January 13, 2021

,

Chapitres

  • 00:04Introduction
  • 00:38Glass Bottom Cell Culture Dish Preparation
  • 01:10Testes Dissection and Mounting
  • 02:31Live Cell In Situ Male Drosophila Germline Stem Cell (GSC) Imaging
  • 04:15Results: Representative Microtubule Time-Lapse Imaging
  • 06:10Conclusion

Summary

Traduction automatique

Presented here is a protocol for super-resolution live-cell imaging in intact tissue. We have standardized the conditions for imaging a highly sensitive adult stem cell population in its native tissue environment. This technique involves balancing temporal and spatial resolution to allow for the direct observation of biological phenomena in live tissue.

Vidéos Connexes

Read Article