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使用转录组学分析绘制无序致癌转录因子的结构-功能关系
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
DOI:

09:58 min

June 27, 2020

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Chapitres

  • 00:04Introduction
  • 00:55In Vitro Construct Panel Setup
  • 02:07Cell Collection
  • 02:47Construct Expression Validation and Correlative Phenotypic Assay Setup
  • 03:50Alignment and Transcript Counting Pipeline
  • 05:32Differential Expression
  • 07:40Results: Representative Disordered Oncogenic Transcription Factor Structure-Function Relationship Mapping
  • 09:31Conclusion

Summary

Traduction automatique

内在无序结构域对致癌融合转录因子功能很重要。为了治疗靶向这些蛋白质,需要更详细地了解这些结构域所采用的调节机制。在这里,我们使用转录组学来绘制尤文氏肉瘤中内在无序的EWS结构域的重要结构特征。

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