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Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling

Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling

DOI:
10.3791/61581-v

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08:58 min

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January 28, 2021

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, , ,
1The James Franck Institute,The University of Chicago, 2Department of Chemistry,The University of Chicago, 3The Institute for Biophysical Dynamics,The University of Chicago

Chapitres

  • 00:04Introduction
  • 00:45Cell-Silicon Nanowire (SiNWs) Hybrid Preparation
  • 04:02Cardiomyocyte (CM):Myofibroblast SiNW Hybrid Co-Culture
  • 05:07Optical Imaging and Stimulation
  • 06:35Results: Representative Intra- and Intercellular Electrical Calcium Coupling Analyses
  • 08:30Conclusion

Summary

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This protocol describes the use of silicon nanowires for intracellular optical bio-modulation of cell in a simple and easy to perform method. The technique is highly adaptable to diverse cell types and can be used for in vitro as well as in vivo applications.

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Keywords: Silicon NanowiresOptical StimulationIntracellular Bioelectrical InterrogationCell SpecificityCardiac CellsBrain CellsElectrical CommunicationMyofibroblastsCardiomyocytesChemical Vapor DepositionNanowire SuspensionCollagen Coating

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