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荧光染料标记脂质体体内组织特异性细胞摄取的制备、给药和评估

Journal JoVE
Bio-ingénierie

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Journal JoVE Bio-ingénierie

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

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荧光染料标记脂质体体内组织特异性细胞摄取的制备、给药和评估

DOI:

10.3791/61585-v

•

08:44 min

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July 30, 2020

•

Victoria Osinski², Alexander L Klibanov³, Coleen A McNamara³

¹Robert M. Berne Cardiovascular Research Center,University of Virginia, ²Department of Pathology,University of Virginia, ³Department of Medicine, Division of Cardiovascular Medicine,University of Virginia

Chapitres

00:05Introduction
00:37Fluorescent Liposome Labeling and Loading
01:59Liposome Administration
02:25Post‐Mortem Tissue Harvest
04:54Adipose Tissue Processing
06:33Results: Liposome Identification, Uptake, and Analysis
08:06Conclusion

Summary

Traduction automatique

该协议的目标是合成荧光标记的脂质体，并使用流式细胞术在细胞水平上鉴定脂质体的体内定位。

Tags

Liposome Preparation Fluorescent Dye Labeling Tesaglitazar In Vivo Cellular Uptake Retro-orbital Injection Blood Sampling Dynamic Light Scattering Spectrophotometry Emulsification Rotary Evaporation Membrane Extrusion

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