Constructing Mutants in Serotype 1 <em>Streptococcus pneumoniae</em> strain 519/43

00:05Introduction
00:35Generation of Plasmid pSD1 and Chemical Transformation of E. coli Dh5α
02:03Plasmid DNA Extraction, Restriction Digestion of pSD1 and Spectinomycin Gene, and Assembly of pSD2
03:03Transformation of S. pneumoniae Strain 519/43
04:46Results: Confirmation of Mutagenesis
05:43Conclusion

Summary

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Here, we describe a S. pneumoniae serotype 1 strain 519/43 that can be genetically modified by using its ability to naturally acquire DNA and a suicide-plasmid. As proof of principle, an isogenic mutant in the pneumolysin (ply) gene was made.

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Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

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