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Mucociliary Epithelial Organoids fra Xenopus embryonale celler: Generation, Kultur og høj opløsning Live Imaging
Journal JoVE
Biologie
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Journal JoVE Biologie
Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging

Mucociliary Epithelial Organoids fra Xenopus embryonale celler: Generation, Kultur og høj opløsning Live Imaging

DOI:
10.3791/61604-v

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07:44 min

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July 28, 2020

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,
1Center for Vascular Research,Institute for Basic Science

Chapitres

  • 00:05Introduction
  • 00:57Isolation of Deep Ectoderm Cells
  • 03:09Generation of Mucociliary Epithelial Organoids
  • 04:29High-resolution Live Imaging of Developing Organoids
  • 05:23Results: Imaging of Developing Organoids
  • 06:53Conclusion

Summary

Traduction automatique

Traduction automatique

Vi beskriver en simpel protokol til at udvikle mucociliære epitelorganoider fra dybe ectoderm celler isoleret fra Xenopus laevis embryoner. De multipotente forfædre regenererer epitelcelleprækursorer og tillader direkte sporing af initieringen og progressionen af celleovergangene på overfladen af organoider.

Tags

Keywords: Mucociliary EpitheliumXenopus Embryonic CellsOrganoid GenerationLive Cell ImagingCell TransitionsRapid ProtocolScalableReproducibleEmbryo CultureAnimal Cap ExcisionDeep Ectoderm Separation

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