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Chapitres
- 00:04Introduction
- 00:47Isolation of Human Peripheral Blood Mononuclear Cells (PBMNCs
- 01:25Activation of Human GPI-Anchored Glycoprotein by Antibody Crosslinking on the Surface of PBMNCs
- 02:21Sorting of Newly Generated Dedifferentiated Cells
- 03:33Preparing Cell Culture Dishes for Neuronal Differentiation of Newly Generated Stem Cells
- 04:10Culturing of Neuronal Differentiated Blood Cells
- 04:44Immunofluorescence Microscopy Analysis of Blood-Derived Neural Cells
- 06:08Results: Morphology, Re-differentiation and Immunophenotyping of Generated Neuronal Cells
- 07:41Conclusion
Vi præsenterer en genetisk modificeret-fri (GM-fri) metode til at opnå celler med en neuronal fænotype fra omprogrammerede perifere blodlegemer. Aktivering af en signalvej, der er knyttet til nyt humant GPI-forbundet protein, afslører en effektiv GM-fri metode til at opnå menneskelige pluripotente stamceller.
