/
/
Isolering av murine spermatogene celler ved hjelp av en violet-spent celle-gjennomtrengelig DNA bindende fargestoff
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology,National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science,Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine,Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science,Tokyo University of Science
Chapitres
- 00:04Introduction
- 00:53Testicular Cell Suspension Preparation
- 02:35Cell Staining
- 03:30Flow Cytometry
- 07:08Results: Representative Spermatogenic Cell Imaging, Yield, and Viability
- 07:55Conclusion
Her presenterer vi en enkel og effektiv metode for å isolere levende meiotiske og postmeiotiske bakterieceller fra voksne musetestikler. Ved hjelp av en lav cytotoksisitet, fiolett-spent DNA bindende fargestoff og fluorescens-aktivert celle sortering, man kan isolere svært beriket spermatogene cellepopulasjoner for mange nedstrøms applikasjoner.
