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Chapitres
- 00:05Introduction
- 01:07Ovarian Cortical Culture Protocol
- 03:15Media Collection, Imaging, and Downstream Processing
- 04:39Results: Analysis of H & E Staining, Number of Follicles at Different Stages, and Collagen Staining of the Ovarian Cortex
- 05:46Conclusion
In vitro culture of bovine ovarian cortex and the effect of nutritional Stair-step diet on ovarian microenvironment is presented. Ovarian cortex pieces were cultured for seven days and steroids, cytokines, and follicle stages were evaluated. The Stair-Step diet treatment had increased steroidogenesis resulting in follicle progression in culture.
