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En Ex Vivo Choroid Spirende Assay af okulær mikrovaskulær angiogenese

Journal JoVE
Biologie

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Journal JoVE Biologie

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

En Ex Vivo Choroid Spirende Assay af okulær mikrovaskulær angiogenese

DOI:

10.3791/61677-v

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06:10 min

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August 06, 2020

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Yohei Tomita, Zhuo Shao, Bertan Cakir, Yumi Kotoda, Zhongjie Fu³, Lois E.H. Smith

¹Department of Ophthalmology,Boston Children’s Hospital, Harvard Medical School, ²Department of Clinical and Metabolic Genetics,Hospital for Sick Children, University of Toronto, ³Manton Center for Orphan Disease,Harvard Medical School, Boston Children’s Hospital

Chapitres

00:04Introduction
00:47Preparation and Experimental Steps
03:26SWIFT-Choroid Computerized Quantification Method
04:25Results: Free Fatty Acid Receptor (FFAR) 4 Suppression Exacerbate Choroidal Neovascularization
05:38Conclusion

Summary

Traduction automatique

Denne protokol præsenterer choroid spiring assay, en ex vivo model af mikrovaskulær spredning. Denne analyse kan bruges til at vurdere veje involveret i prolifererende choroidal mikro fartøjer og vurdere narkotika behandlinger ved hjælp af vilde type og genetisk modificerede musevæv.

Tags

Keywords: Ex Vivo Choroid Angiogenesis Microvascular Age-related Macular Degeneration (AMD)Choroidal Neovascularization BME (basal Membrane Extract)Classic Medium Microscopy

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