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En ex vivo choroid spirende analyse av okulær mikrovaskulær angiogenese
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Biologie
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Journal JoVE Biologie
An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

En ex vivo choroid spirende analyse av okulær mikrovaskulær angiogenese

DOI:
10.3791/61677-v

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06:10 min

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August 06, 2020

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, , , , ,
1Department of Ophthalmology,Boston Children’s Hospital, Harvard Medical School, 2Department of Clinical and Metabolic Genetics,Hospital for Sick Children, University of Toronto, 3Manton Center for Orphan Disease,Harvard Medical School, Boston Children’s Hospital

Chapitres

  • 00:04Introduction
  • 00:47Preparation and Experimental Steps
  • 03:26SWIFT-Choroid Computerized Quantification Method
  • 04:25Results: Free Fatty Acid Receptor (FFAR) 4 Suppression Exacerbate Choroidal Neovascularization
  • 05:38Conclusion

Summary

Traduction automatique

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Denne protokollen presenterer choroid spirende analyse, en ex vivo modell av mikrovaskulær spredning. Denne analysen kan brukes til å vurdere veier involvert i å spre koroide mikrokar og vurdere medikamentell behandling ved hjelp av vill type og genmodifisert musevev.

Tags

Keywords: Ex VivoChoroidAngiogenesisMicrovascularAge-related Macular Degeneration (AMD)Choroidal NeovascularizationBME (basal Membrane Extract)Classic MediumMicroscopy

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