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Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues
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Journal JoVE Biologie
Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues

Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues

DOI:
10.3791/61694-v

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06:17 min

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August 25, 2020

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,
1Department of Biochemistry and Molecular Biology,Mayo Clinic

Chapitres

  • 00:04Introduction
  • 00:34Dissection of Epididymal Fat Pad and Tissue Dissociation
  • 02:38Antibody Labeling and Fluorescence Activated Cell Sorting (FACS)
  • 04:48Results: Isolation of Viable Sca1+ Adipose Progenitor Single Cells
  • 05:34Conclusion

Summary

Traduction automatique

Traduction automatique

We present a simple method to isolate highly viable adipose progenitor cells from mouse epididymal fat pads using fluorescence activated cell sorting.

Tags

Adipose Progenitor CellsCell IsolationCell SeparationFlow CytometrySca1Ter119CD31CD45

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