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Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
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Chimie
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Journal JoVE Chimie
Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

DOI:
10.3791/61728-v

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08:51 min

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September 15, 2020

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, , ,
1Centre for Biospectroscopy and School of Chemistry,Monash University, 2Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology,Monash University, 3Department of Infectious Diseases, The Alfred Hospital and Central Clinical School,Monash University

Chapitres

  • 00:05Introduction
  • 00:53Atomic Force Microscopy-Infrared (AFM-IR) Spectroscopy Sample Preparation
  • 02:50Instrument Preparation
  • 03:50Single Spectrum Data Collection
  • 05:42Imaging Approach Data Collection
  • 06:41Results: Representative AFM-IR Analyses of Single Bacterium Chemistry
  • 08:11Conclusion

Summary

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Traduction automatique

Atomic Force Microscopy-Infrared Spectroscopy (AFM-IR) provides a powerful platform for bacterial studies, enabling to achieve nanoscale resolution. Both, mapping of subcellular changes (e.g., upon cell division) as well as comparative studies of chemical composition (e.g., arising from drug resistance) can be conducted at a single cell level in bacteria.

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AI WriterAFM-IRNanoscale AnalysisBacteriaAntimicrobial ResistanceSample PreparationAFM-IR Spectroscopy

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