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Biologie du développement
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4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy
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Biologie du développement
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Journal JoVE Biologie du développement
4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

DOI:
10.3791/61736-v

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08:38 min

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October 08, 2020

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, , , , ,
1CIBIR (Center for Biomedical Research of La Rioja), La Rioja, Spain, 2IBIS (Instituto de Biomedicina de Sevilla),Hospital Universitario Virgen del Rocío/CSIC/ Universidad de Sevilla, Sevilla, Spain

Chapitres

  • 00:04Introduction
  • 01:114D Microscope and Slide Preparation
  • 02:28Embryo Mounting
  • 03:364D Microscope 3D Image Acquisition
  • 04:254D Movie Analysis
  • 05:28Cell Lineage Tracing
  • 06:55Results: Representative C. Elegans Cell Lineage Tracing and Embryonic Development Evaluation
  • 07:58Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol for preparing and mounting Caenorhabditis elegans embryos, recording development under a 4D microscope and tracing cell lineage.

Tags

4D MicroscopyCaenorhabditis ElegansNomarski MicroscopyCell Lineage TracingCell CyclingProliferationDifferentiationApoptosisAgar PadEmbryonic Development

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