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Immunologie et infection
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Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
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Immunologie et infection
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Journal JoVE Immunologie et infection
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

DOI:
10.3791/61749-v

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07:24 min

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February 06, 2021

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, , , , ,
1Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology,Dean McGee Eye Institute, 3Dean McGee Eye Institute, 4Department of Cell Biology and Department of Family and Preventive Medicine,University of Oklahoma Health Sciences Center

Chapitres

  • 00:04Introduction
  • 00:54Bacillus cereus Culture and Dilution
  • 01:48Mouse Intravitreal Injection
  • 04:02Eye Harvest
  • 04:43Intraocular Bacterial Count
  • 06:06Results: Representative Bacterial Endophthalmitis Mouse Model Validation
  • 06:47Conclusion

Summary

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Traduction automatique

We describe here a method of intravitreal injection and subsequent bacterial quantitation in mouse model of bacterial endophthalmitis. This protocol can be extended for measuring host immune responses and bacterial and host gene expression.

Tags

Keywords: Intravitreal InjectionMouse ModelBacterial EndophthalmitisPathogenesisIntraocular InfectionQuantitationMicroorganismsHost Immune ResponsesGene ExpressionTreatment EffectivenessBiosafety Level TwoB. CereusMicroinjectorOphthalmic MicroscopeAnesthetized MouseReverse Action Forceps

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