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1Cardiovascular Research Institute for Intractable Disease, College of Medicine,The Catholic University of Korea, 2Division of Cardiology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, 3Division of Cardiology, Department of Internal Medicine, Uijeongbu St. Mary’s Hospital, College of Medicine,The Catholic University of Korea
Chapitres
- 00:05Introduction
- 01:49Preparation of MSCs and Injectable Gelatin Hydrogels
- 03:38In Situ MSC-loading and Three-dimensional in Vitro Culture
- 04:29Induction of Myocardial Infarction in Mice
- 05:44Intramyocardial Transplantation of MSC-loading GH Hydrogels
- 06:37Results: Improving Stem Cell Retention and Engraftment with Injectable Hydrogels
- 08:31Conclusion
La terapia a base di cellule staminali è emersa come una strategia efficiente per riparare i tessuti cardiaci feriti dopo l'infarto del miocardio. Forniamo un'applicazione in vivo ottimale per il trapianto di cellule staminali utilizzando idrogel di gelatina che possono essere enzimaticamente incrociati.
Tags
Mesenchymal Stem CellsMSCsInjectable HydrogelsIntramyocardial TransplantationMyocardial InfarctionMurine ModelCell RetentionCell SurvivalGrowth FactorsGenetic MaterialsDrugsIn Vitro ExperimentIn Vivo ExperimentCell CultureCell SuspensionTrypsinTrypan BlueCell CountingGH Conjugate SolutionHydrogen PeroxideDual Syringe
