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Chapitres
- 00:04Introduction
- 00:46Array Generation
- 01:42Sample Sectioning
- 02:23Array Sectioning and Transfer
- 03:53Scanning Electron Microscopy (SEM)
- 04:16Imaging Region Definition
- 06:37Auto Function Configuration and Image Acquisition
- 07:27Results: Representative Targeted Acquisition of Volume Information
- 09:02Conclusion
We describe the preparation of ribbons of serial sections and their collection on large transfer support for use as Array Tomography samples, along with automated imaging procedures in a scanning electron microscope. The protocol allows screening, retrieval, and targeted imaging of local, rare events, and the acquisition of large data volumes.
