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Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
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Journal JoVE Ingénierie
Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

DOI:
10.3791/61862-v

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08:03 min

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April 13, 2022

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1NSD, Oak Ridge National Laboratory

Chapitres

  • 00:04Introduction
  • 01:17Measurement of NSE
  • 03:58NSE Data Reduction
  • 05:05NSE Data Fitting
  • 05:33Results: NSE Relaxation Spectra for IgG and MBP Proteins
  • 06:39Conclusion

Summary

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The present protocol describes methods for investigating the structure and dynamics of two model proteins that have an important role in human health. The technique combines bench-top biophysical characterization with neutron spin echo spectroscopy to access the dynamics at time and length scales relevant for protein interdomain motions.

Tags

Neutron Spin Echo SpectroscopyProtein DynamicsProtein Domain MotionsBiophysical ResearchSample PreparationSample LoadingSample AlignmentTemperature ControlData Collection

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